Abstract:
Milk is an intricate or a complex biological fluid and by its nature, a good grawthmedium for 
many microorganisms.Becouse of its physico-chemical properties, its needs strict hygienic 
condition to avoid contamination of milk with microorganisms.Therefore, the microbial 
content of milk is a major feature in determining its quality.Microbial contamination can 
generally occure from three sources such as, with in the udder, exterior of the udder and 
surface of milk handling and storage equipment. Thus, this study was carried out to assess the 
microbiological quality of raw caw milk and yoghurt sample collected from household and 
vendors in Haramaya woreda.A total of 27 milk samples was collected from household and 
venders and transported to the laboratoryat <4℃ for total plate count, Coliformcounts, 
Enterobacteriaceaecounts, and yeastand mold counts determination. Mean total plate 
count,Coliform count, Entrobacteriaceae count,Yeast and mold count for raw milk samples 
collected from the households were 5.97±0.88,4.96±0.70, 4.54±0.72,and 3.9±0.64 and 
4.1±0.66log10 cfu/ml, respectively. The mean values for raw milk samples collected from the 
equipment of the vendors were 6.5±0.77, 5.0±0.81, 4.2±0.60, and 3.5±0.4 and 3.6±0.43log10 
cfu/ml for total plate count,Coliform count,Entrobacteriaceae count,Yeast and mold 
countsrespectively.The mean total plate count,Coliform count,Entrobacteriaceae count,Yeast 
and mold count for yoghurt sample collected from the equipment of the household were
5.74±0.51, 4.9±0.7, 4.54±0.85, 4.4±0.71 and 4.5±0.83log10 cfu/ml respectively.The microbial 
counts for samples collected from the equipment of the vendors were 6.2±1.34, 4.2±0.63, 
4.2±0.77±, 4.35±0.07 and 4.2±0.14log10 cfu/ml for total plate count,Coliform count, 
Entrobacteriaceae count,Yeast and mold count, respectively.To protect the raw and fermented 
cow milk from spoilage loss and consumers from milk born public health risk, the availability 
of documented information on the microbiological quality of raw and fermented caw milk 
across the milk supply chain is critically important as such information may be important for 
different organization to undertake relevant development intervention on hygienic practices 
essential for safe milk production and handling.Generally, the present study revealed that 
milk samples contained higher microbial load than different standards and considered as 
substandard which will result in public health hazard to the consumer.Therefore, intensive 
study on microbial status of milk in the study sites should be conducted to assure safety and 
quality policies to be set to assure the supply of quality milk in the area.It could be concluded 
that raw and yoghurt milk samples collected from household and vendors were severely 
contaminated with total bacterial count, coliform count,entrobacterial count, yeast and 
molds, with exceeding the respective acceptable limits.